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anti phospho epha2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti phospho epha2
    Anti Phospho Epha2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+phospho+epha2/Phospho-EphA2+(Ser897)+Rabbit+mAb/pmc12972985-265-76-82
    Average 95 stars, based on 125 article reviews
    anti phospho epha2 - by Bioz Stars, 2026-09
    95/100 stars

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    other:

    Article Title: Phosphotyrosine proteomics in cells synchronized at monopolar cytokinesis reveals EphA2 as functioning in cytokinesis.
    Article Snippet: Cytokinesis is the final step of the cell division in which cellular components are separated into two daughter cells.. This process is regulated through the phosphorylation of different classes of proteins by serine/threonine (Ser/Thr) kinases such as Aurora B and Polo-like kinase 1 (PLK1).. Conversely, the role of phosphorylation at tyrosine residues during cytokinesis has not been studied in detail yet.

    Western Blot:

    Article Title: EphA2 is an epithelial cell pattern recognition receptor for fungal β-glucans
    Article Snippet: .. The lysates were separated by SDS/PAGE, and the phosphorylated proteins were detected by immunoblotting with phospho-specific antibodies, including anti-phospho-EphA2 (Cell signaling; #6347), anti-phospho-Stat3 (Cell signaling; #9134), anti-phospho-c-Fos (Cell signaling; #5348), anti-phospho-MEK1/2 (Cell signaling; #9154), anti-phospho-p65 (Cell signaling; # 3033). .. The blots were then stripped, and total protein levels and β-actin were detected by immunoblotting with appropriate antibodies against EphA2 (Cell signaling; #6997), EphB2 (Cell signaling; #83029), EphA4 (Santa Cruz; sc-365503), Stat3 (Cell signaling; # 12640), c-Fos (Cell signaling; # 4384), MEK1/2 (Cell signaling; # 9122), p65 (Cell signaling; # 8242), and β-actin (Cell signaling # 3700).

    Article Title: EphA2 is an epithelial cell pattern recognition receptor for fungal β-glucans.
    Article Snippet: .. The lysates were separated by SDS-PAGE, and the phosphorylated proteins were detected by immunoblotting with phospho-specific antibodies, including anti-phospho-EphA2 (Cell Signaling; #6347), anti-phospho-Stat3 (Cell Signaling; #9134), anti-phosphoc-Fos (Cell Signaling; #5348), anti-phospho-MEK1/2 (Cell Signaling; #9154) and anti-phospho-p65 (Cell Signaling; # 3033).The blots were then stripped, and total protein levels and β -actin were detected by immunoblotting with appropriate antibodies against EphA2 (Cell Signaling; #6997), EphB2 (Cell Signaling; #83029), EphA4 (Santa Cruz Biotechnology; sc-365503), Stat3 (Cell Signaling; # 12640), c-Fos (Cell Signaling; # 4384), MEK1/2 (Cell Signaling; # 9122), p65 (Cell Signaling; # 8242) and β -actin (Cell Signaling; # 3700). .. The blots were developed using enhanced chemiluminescence and imaged with either a FluorChem 8900 (Alpha Innotech, San Leandro, CA, USA) or C400 (Azure Biosystems, Dublin, CA, USA) digital imager.

    Article Title: Targeting EphA2 under DNA damage causes mitotic bypass via p21 induction
    Article Snippet: For Ca Ski cells, transfection was performed using PEI MAX (24765, Polysciences), and cells were selected with 2 μg/ml blasticidin S for 14 days. .. Primary antibodies used for immunoblotting (IB), immunofluorescence (IF) or flow cytometry were as follows: mouse monoclonal anti-Chk1 (IB, 1:1000; G-4, sc-8408, Santa Cruz Biotechnology), anti-γ-tubulin (IF, 1:250; GTU-88, T6557, Merck), anti-p21 (IB, 1:1000, DCS60, 2946, Cell Signaling Technology), anti-p53 (IB, 1:1000; DO-1, sc-126, Santa Cruz Biotechnology), and anti-phospho-histone H3 (IF, 1:400; 6G3, 9706, Cell Signaling Technology) antibodies; rabbit monoclonal anti-EphA2 (IB, 1:1000; 6997S, Cell Signaling Technology), anti-phospho-Chk1 (Ser345, IB, 1:1000; 133D3, #2348, Cell Signaling Technology), and anti-phospho-EphA2 (Ser897, IB, 1:1000; D9A1, 6347, Cell Signaling Technology) antibodies; rabbit polyclonal anti-cyclin B1 (IB, 1:3000; IF and flow cytometry, 1:250; H-433, sc-752, Santa Cruz Biotechnology), anti-phospho-histone H2A.X (γH2AX, IB, 1:500; 2577S, Cell Signaling Technology), and anti-phospho KAP1 (Ser824, IB, 1:1000; A300–767A, Bethyl Laboratories, Montgomery) antibodies; and rat monoclonal anti-α-tubulin (IB, 1:4000; IF, 1:800; MCA78 G, Bio-Rad) antibody. .. For immunoblotting analysis, horseradish peroxidase-conjugated donkey anti-mouse IgG (1:8000; 715–035–151), donkey anti-rabbit IgG (1:8000; 711–035–152), and donkey anti-rat IgG (1:8000–10000; 712–035–153) antibodies were purchased from Jackson ImmunoResearch Laboratories and used as secondary antibodies.

    Article Title: EphA2 Is a Lung Epithelial Cell Receptor for Pneumocystis β-Glucans.
    Article Snippet: .. Total protein, 5 μg per sample, was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the phosphorylated EphA2 protein detected by immunoblotting with the phosphatase-specific antibody, anti-phospho-EphA2 (Cell Signaling; number 6347). .. Total EphA2 and β2 microglobulin (B2M) were detected with appropriate antibodies, EphA2 (Cell Signaling; number 6997) and B2M (Cell Signaling; number 59035), respectively.

    Nucleic Acid Electrophoresis:

    Article Title: Cytomegalovirus US28 regulates cellular EphA2 to maintain viral latency
    Article Snippet: .. Equal amounts of protein per sample were separated by SDS–polyacrylamide gel electrophoresis, and proteins were detected using the following antibodies (table S3): streptavidin–horseradish peroxidase (HRP, Thermo Fisher Scientific), anti-FLAG clone M2 (Millipore Sigma, 1:7500), anti-HA clone 3F10 (Millipore Sigma, 1:1000), anti–phospho-Fos (Ser 32 ) [Cell Signaling Technology (CST), 1:1000], anti–c-Fos (CST, 1:1000), anti–phospho-p44/42 MAPK (ERK1/2; Thr 202 /Tyr 204 ) (CST, 1:1000), anti-p44/42 MAPK (ERK1/2) (CST, 1:1000), anti–phospho-Src (Tyr 527 ) (CST, 1:1000), anti–phospho-Src (Tyr 416 ) (CST, 1:1000), anti-Src (CST, 1:1000), anti–phospho-MEK1/2 (CST, 1:1000), anti-MEK1/2 (CST, 1:1000), anti–phospho-EphA2 (Ser 897 ) (CST, 1:1000), anti-EphA2 (CST, 1:1000), anti-CMV IE1 [clone 1B12, 1:100, ( )], anti–β-actin peroxidase (Millipore Sigma, 1:20,000), and goat anti-mouse, goat anti-rat, and goat anti-rabbit HRP secondary antibodies (all from Jackson ImmunoResearch Labs; 1:10,000). ..

    Immunofluorescence:

    Article Title: Targeting EphA2 under DNA damage causes mitotic bypass via p21 induction
    Article Snippet: For Ca Ski cells, transfection was performed using PEI MAX (24765, Polysciences), and cells were selected with 2 μg/ml blasticidin S for 14 days. .. Primary antibodies used for immunoblotting (IB), immunofluorescence (IF) or flow cytometry were as follows: mouse monoclonal anti-Chk1 (IB, 1:1000; G-4, sc-8408, Santa Cruz Biotechnology), anti-γ-tubulin (IF, 1:250; GTU-88, T6557, Merck), anti-p21 (IB, 1:1000, DCS60, 2946, Cell Signaling Technology), anti-p53 (IB, 1:1000; DO-1, sc-126, Santa Cruz Biotechnology), and anti-phospho-histone H3 (IF, 1:400; 6G3, 9706, Cell Signaling Technology) antibodies; rabbit monoclonal anti-EphA2 (IB, 1:1000; 6997S, Cell Signaling Technology), anti-phospho-Chk1 (Ser345, IB, 1:1000; 133D3, #2348, Cell Signaling Technology), and anti-phospho-EphA2 (Ser897, IB, 1:1000; D9A1, 6347, Cell Signaling Technology) antibodies; rabbit polyclonal anti-cyclin B1 (IB, 1:3000; IF and flow cytometry, 1:250; H-433, sc-752, Santa Cruz Biotechnology), anti-phospho-histone H2A.X (γH2AX, IB, 1:500; 2577S, Cell Signaling Technology), and anti-phospho KAP1 (Ser824, IB, 1:1000; A300–767A, Bethyl Laboratories, Montgomery) antibodies; and rat monoclonal anti-α-tubulin (IB, 1:4000; IF, 1:800; MCA78 G, Bio-Rad) antibody. .. For immunoblotting analysis, horseradish peroxidase-conjugated donkey anti-mouse IgG (1:8000; 715–035–151), donkey anti-rabbit IgG (1:8000; 711–035–152), and donkey anti-rat IgG (1:8000–10000; 712–035–153) antibodies were purchased from Jackson ImmunoResearch Laboratories and used as secondary antibodies.

    Flow Cytometry:

    Article Title: Targeting EphA2 under DNA damage causes mitotic bypass via p21 induction
    Article Snippet: For Ca Ski cells, transfection was performed using PEI MAX (24765, Polysciences), and cells were selected with 2 μg/ml blasticidin S for 14 days. .. Primary antibodies used for immunoblotting (IB), immunofluorescence (IF) or flow cytometry were as follows: mouse monoclonal anti-Chk1 (IB, 1:1000; G-4, sc-8408, Santa Cruz Biotechnology), anti-γ-tubulin (IF, 1:250; GTU-88, T6557, Merck), anti-p21 (IB, 1:1000, DCS60, 2946, Cell Signaling Technology), anti-p53 (IB, 1:1000; DO-1, sc-126, Santa Cruz Biotechnology), and anti-phospho-histone H3 (IF, 1:400; 6G3, 9706, Cell Signaling Technology) antibodies; rabbit monoclonal anti-EphA2 (IB, 1:1000; 6997S, Cell Signaling Technology), anti-phospho-Chk1 (Ser345, IB, 1:1000; 133D3, #2348, Cell Signaling Technology), and anti-phospho-EphA2 (Ser897, IB, 1:1000; D9A1, 6347, Cell Signaling Technology) antibodies; rabbit polyclonal anti-cyclin B1 (IB, 1:3000; IF and flow cytometry, 1:250; H-433, sc-752, Santa Cruz Biotechnology), anti-phospho-histone H2A.X (γH2AX, IB, 1:500; 2577S, Cell Signaling Technology), and anti-phospho KAP1 (Ser824, IB, 1:1000; A300–767A, Bethyl Laboratories, Montgomery) antibodies; and rat monoclonal anti-α-tubulin (IB, 1:4000; IF, 1:800; MCA78 G, Bio-Rad) antibody. .. For immunoblotting analysis, horseradish peroxidase-conjugated donkey anti-mouse IgG (1:8000; 715–035–151), donkey anti-rabbit IgG (1:8000; 711–035–152), and donkey anti-rat IgG (1:8000–10000; 712–035–153) antibodies were purchased from Jackson ImmunoResearch Laboratories and used as secondary antibodies.

    Polyacrylamide Gel Electrophoresis:

    Article Title: EphA2 Is a Lung Epithelial Cell Receptor for Pneumocystis β-Glucans.
    Article Snippet: .. Total protein, 5 μg per sample, was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the phosphorylated EphA2 protein detected by immunoblotting with the phosphatase-specific antibody, anti-phospho-EphA2 (Cell Signaling; number 6347). .. Total EphA2 and β2 microglobulin (B2M) were detected with appropriate antibodies, EphA2 (Cell Signaling; number 6997) and B2M (Cell Signaling; number 59035), respectively.



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